gfap (Novus Biologicals)
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93
Structured Review
Novus Biologicals
gfap
Gfap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfap/GFAP+Antibody/pmc13061562-88-21-23
Average 93 stars, based on 38 article reviews
Gfap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfap/GFAP+Antibody/pmc13061562-88-21-23
Average 93 stars, based on 38 article reviews
gfap - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Stroke Exacerbates Respiratory Disorder and Cognition Impairment in Mice with Cerebral Amyloid Angiopathy"
Article Title: Stroke Exacerbates Respiratory Disorder and Cognition Impairment in Mice with Cerebral Amyloid Angiopathy
Journal: Aging and Disease
doi: 10.14336/AD.2025.0474
Figure Legend Snippet: Stroke increases astrocytic gliosis and induced astrocytic polarization in RTN. (A) RTN chemo-sensitive phox2B-positive neurons (area circumscribed by red solid line) are located ventromedial to the VII, medial to the pyramidal tract. (B) Histological representation of the RTN location within the brainstem, illustrating the specific area analyzed in both WT, CAA, and stroke mice, in relation to the 7th Facial Nucleus (7 N), Scale bar = 300μm/50 μm (zoom). (C-G) Images show the GFAP expression in each group(F (2,18) =19.043, P ＜0.001), and dual-IF staining for GFAP with C3 (F (2,18) =19.851, P ＜0.001 ), GFAP with S100A10 (F (2,18) =21.306, P ＜0.001) in RTN from WT, CAA, and stroke mice at day 42 post-ischemia (n= 5 for WT group, n=7 for CAA-Sham group and n=9 for CAA-pd-MCAO group). Scale bar = 50 µm. * P <0.05; ** P <0.01; *** P <0.001. ns: non-significance. The data are shown as the mean ± SEM. After performing the Shapiro-Wilk normality test to examine the normal distribution, One-way analysis of variance (ANOVA) was employed to analyze the data pertaining to multiple groups, subsequently, multiple comparisons were conducted using the uncorrected Fisher's LSD test.
Techniques Used: Expressing, Staining
Related Articles
Blocking Assay:Article Title: CCR2/CCR5 antagonism with cenicriviroc relieves neuropathic pain induced by sciatic nerve injury and delays morphine tolerance in mice. Article Snippet: Next, the proteins were transferred to Immune-Blot PVDF membranes (Bio-Rad) with a semidry transfer system (30 min, 25 V), and then the membranes were blocked for 1 h at RT with 5% bovine serum albumin (Merck) in Tris-buffered saline containing 0.1% Tween-20 (TBST). .. After blocking, the membranes were washed with TBST (1 × 2 min and 1 × 5 min) and incubated overnight (4 ◦ C) with the following commercially available primary antibodies: rabbit: anti-CCR2 (1:500, NBP1-48337; Novus Biologicals; Centennial, USA), anti-CCR5 (1:500, NBP2-31374; Novus Biologicals), anti-p-MOR^S363 (1:500, 7TM0319A; 7TM Antibodies, Jena, Germany), anti-p-DOR^S363 (1:500, bs-3712R; Bioss Antibodies, Woburn, MA, USA), anti-IBA1 (1:500, NBP2-19019; Novus Biologicals), Incubation:Article Title: CCR2/CCR5 antagonism with cenicriviroc relieves neuropathic pain induced by sciatic nerve injury and delays morphine tolerance in mice. Article Snippet: Next, the proteins were transferred to Immune-Blot PVDF membranes (Bio-Rad) with a semidry transfer system (30 min, 25 V), and then the membranes were blocked for 1 h at RT with 5% bovine serum albumin (Merck) in Tris-buffered saline containing 0.1% Tween-20 (TBST). .. After blocking, the membranes were washed with TBST (1 × 2 min and 1 × 5 min) and incubated overnight (4 ◦ C) with the following commercially available primary antibodies: rabbit: anti-CCR2 (1:500, NBP1-48337; Novus Biologicals; Centennial, USA), anti-CCR5 (1:500, NBP2-31374; Novus Biologicals), anti-p-MOR^S363 (1:500, 7TM0319A; 7TM Antibodies, Jena, Germany), anti-p-DOR^S363 (1:500, bs-3712R; Bioss Antibodies, Woburn, MA, USA), anti-IBA1 (1:500, NBP2-19019; Novus Biologicals), Article Title: Optic Nerve Head Spatial Transcriptomic Change in Nonhuman Primate Early Experimental Glaucoma Article Snippet: .. After stringent washes, samples were incubated with anti-Iba1 (E4O4W, XP Rabbit mAb, Alexa Fluor 594 Conjugate, #48934, dilution 1:200), Article Title: Optic Nerve Head Spatial Transcriptomic Change in Nonhuman Primate Early Experimental Glaucoma. Article Snippet: .. After stringent washes, samples were incubated with anti-Iba1 (E4O4W, XP Rabbit mAb, Alexa Fluor 594 Conjugate, #48934, dilution 1:200), Staining:Article Title: Optic Nerve Head Spatial Transcriptomic Change in Nonhuman Primate Early Experimental Glaucoma Article Snippet: .. After stringent washes, samples were incubated with anti-Iba1 (E4O4W, XP Rabbit mAb, Alexa Fluor 594 Conjugate, #48934, dilution 1:200), Article Title: Optic Nerve Head Spatial Transcriptomic Change in Nonhuman Primate Early Experimental Glaucoma. Article Snippet: .. After stringent washes, samples were incubated with anti-Iba1 (E4O4W, XP Rabbit mAb, Alexa Fluor 594 Conjugate, #48934, dilution 1:200), other:Article Title: Systemic inflammation triggers local complement production in the mouse retina and RPE. Article Snippet: Volcano plots to show the up and down regulated proteins was created using GraphPad Prism software version 9.5.1 (San Diego, CA, USA). |